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  • 赵自通,郝莉花,李宇健,陈复生.复合植物水解酶提取花生油脂体工艺研究[J].食品科学技术学报,2017,35(3):31-35.    [点击复制]
  • ZHAO Zitong,HAO Lihua,LI Yujian,CHEN Fusheng.Optimization of Enzyme-Assisted Aqueous Extraction for Peanut Oil Body[J].Journal of Food Science and Technology,2017,35(3):31-35.   [点击复制]
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复合植物水解酶提取花生油脂体工艺研究
赵自通,郝莉花,李宇健,陈复生
0
(河南工业大学 粮油食品学院, 河南 郑州 450001)
摘要:
为了提高花生油脂体的提取率,选用复合植物水解酶辅助提取花生油脂体。通过单因素实验考察了料液比、酶用量、酶解时间、酶解温度对复合植物水解酶提取花生油脂体提取率的影响。在单因素实验的基础上,采用正交试验对复合植物水解酶提取花生油脂体的工艺进行优化。实验结果表明,在所选取的参数范围内,各因素对花生油脂体提取率的影响由大到小为:料液比、酶解温度、酶解时间、酶用量。获得其较佳工艺条件为料液比1∶4 (g/mL)、酶用量1.25%、酶解时间80min、酶解温度50℃,在该条件下花生油脂体提取率为48.92%。
关键词:  花生  油脂体  复合植物水解酶  提取率
DOI:10.3969/j.issn.2095-6002.2017.03.004
投稿时间:2017-04-09
基金项目:国家自然科学基金资助项目(21376064;21676073);国家“863”计划项目(2013AA102208);“粮油食品加工与新技术研究”河南省优秀科技创新团队项目;中国博士后科学基金资助项目(2015M582184)。
Optimization of Enzyme-Assisted Aqueous Extraction for Peanut Oil Body
ZHAO Zitong,HAO Lihua,LI Yujian,CHEN Fusheng
(College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China)
Abstract:
In order to improve the extraction rate of peanut oil bodies, Viscozyme L. was used to assist the extraction of peanut oleosome. The effects of material ratio, enzyme concentration, incubation time, and incubation temperature on extraction rate of peanut oil body with compound plant hydrolase were investigated by using the single factor experiment. On the basis of single factor experiment, the orthogonal experiment was used to optimize the process conditions of extracting peanut oil body with compound plant hydrolase. The results showed that the influence order from high to low of the factors was material ratio, incubation temperature, incubation time, enzyme concentration. The optimum conditions were as follows:material ratio 1∶4 (g/mL), enzyme concentration 1.25%, the incubation time 80min and the incubation temperature 50℃. Under the optimum conditions, the extraction rate of peanut oil body was 48.92%.
Key words:  peanut  oil body  compound plant hydrolase  yield