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  • 赵丰丽,吴奔,张昌志,范彩琴,宋云飞,杨文国,陈静.罗汉果内生菌及其周边土壤中产α-淀粉酶菌株的筛选[J].食品科学技术学报,2017,35(3):61-65, 94.    [点击复制]
  • ZHAO Fengli,WU Ben,ZHANG Changzhi,FAN Caiqin,SONG Yunfei,YANG Wenguo,CHEN Jing.Screening of α-Amylase Producing Strains from Endophytic Bacteria in Siraitia grosvenorii and Its Surrounding Soil[J].Journal of Food Science and Technology,2017,35(3):61-65, 94.   [点击复制]
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罗汉果内生菌及其周边土壤中产α-淀粉酶菌株的筛选
赵丰丽,吴奔,张昌志,范彩琴,宋云飞,杨文国,陈静
0
(广西师范大学 生命科学学院, 广西 桂林 541004;珍稀濒危动植物生态与环境保护教育部重点实验室, 广西 桂林 541004;桂林莱茵生物科技股份有限公司, 广西 桂林 541199)
摘要:
对罗汉果内生菌及其种植区土壤中的微生物进行分离,筛选淀粉酶产生菌,并研究其发酵条件,结果得到15株内生菌和8株微生物,从中筛选到2株产淀粉酶高的菌株2-1(土壤)和R-4(根部内生菌)。经初步鉴定,菌株2-1为球菌,菌株R-4为芽孢杆菌,均为革兰氏阳性菌。研究显示,菌株2-1产酶条件为1.0%氯化铵、0.5%酵母粉、1.0%可溶性淀粉、0.4%氯化钠,发酵温度为40℃,发酵3d,其产酶量为127.65U/mL;R-4菌株产酶条件为0.5%酵母粉、1.0%氯化铵、0.05%氯化钠、2.0%可溶性淀粉,发酵温度为40℃,发酵3d,其产酶量为197.21U/mL。
关键词:  罗汉果  内生真菌  α-淀粉酶  发酵条件
DOI:10.3969/j.issn.2095-6002.2017.03.009
投稿时间:2016-06-29
基金项目:桂林莱茵生物科技股份有限公司与广西师范大学合作项目;广西科学研究与技术开发计划项目(桂科重14121001-4-3)。
Screening of α-Amylase Producing Strains from Endophytic Bacteria in Siraitia grosvenorii and Its Surrounding Soil
ZHAO Fengli,WU Ben,ZHANG Changzhi,FAN Caiqin,SONG Yunfei,YANG Wenguo,CHEN Jing
(College of Life Science, Guangxi Normal University, Guilin 541004,China;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin 541004,China;Guilin Layn Natural Ingredients Corp., Guilin 541199, China)
Abstract:
In this study, the screening of α-amylase producing strains from endophytic bacteria in Siraitia grosvenorii and surrounding soil were preformed, and the optimum fermentation conditions for α-amylase producing strains were performed. Our results showed that 15 strains were isolated from the Siraitia grosvenori and 8 strains from surrounding soil. High amylase producing strains 2-1 (surrounding soil) and R-4 (root endophytic bacteria) were isolated. The identification results showed that 2-1 strain was staphylococcus and R-4 strain was bacillus, which were gram positive bacteria. The appropriate condition of enzyme production for strains 2-1 and R-4 were determined. For strain 2-1, the appropriate conditions were 1.0% ammonium chloride, 0.5% yeast powder, 1.0% soluble starch, 0.4% sodium chloride, fermentation temperature 40℃, and fermentation time 3 days and the enzyme production was 127.65U/mL under these conditions. For strain R-4, the appropriate conditions were 0.5% yeast powder, 1.0% ammonium chloride, 0.05% sodium chloride, 2.0% soluble starch, fermentation temperature 40℃, and fermentation time 3 days, and the enzyme production was 197.21U/mL under these conditions.
Key words:  Siraitia grosvenorii  endophytic bacteria  α-amylase  fermentation condition