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  • 李文,李民赞,孙明.菠菜中乐果农药残留的比色法测定条件研究[J].食品科学技术学报,2013,31(6):58-62.    [点击复制]
  • LI Wen,LI Min-zan,SUN Ming.Study on the Colorimetry Determination Conditions of Rogor Pesticide Residues in Spinage[J].Journal of Food Science and Technology,2013,31(6):58-62.   [点击复制]
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菠菜中乐果农药残留的比色法测定条件研究
李文,李民赞,孙明
0
(北京工商大学 计算机与信息工程学院;中国农业大学 现代精细农业系统集成研究教育部重点实验室)
摘要:
为研究菠菜中乐果农药残留的快速安全检测方法,根据乐果在碱性-NaOH-溶液中分解,生成物Na2S和亚硝酰铁氰化钠反应生成洋红色产物的原理,对比色条件进行了研究和优化. 详细分析并验证了显色体系的配制方法,确定了显色体系的较佳参数值:pH值为5 7,温度为24 ℃, 反应时间为20 s. 实验结果表明,最佳比色条件下在390 nm处有明显的特征吸收峰,可以测定1×10 -6的乐果农药残留. 用最小二乘法建立了菠菜中乐果农药残留的数学模型,相关系数 R 2=0 941 5,样品回收率为91 6%~108 9%,为以后开发便携式农药残留检测仪器的光源提供了依据.
关键词:  乐果农药残留  比色法  光谱分析
DOI:10.3969/j.issn.2095-6002.2013.06.011
基金项目:公益性行业-农业-科研专项资助项目-201003008-
Study on the Colorimetry Determination Conditions of Rogor Pesticide Residues in Spinage
LI Wen,LI Min-zan,SUN Ming
(College of Computer Science and Information Engineering, Beijing Technology and Business University;Key Laboratory of Modern Precision Agriculture System Integration Research of Ministry of Education, China Agricultural University)
Abstract:
In order to research the fast and safe determination method of Rogor pesticide residues in spinage, the colorimetric reaction conditions were researched and optimized in this study according to the principle that Nitrosyl iron sodium cyanide can produce carmine product by reacting with Na 2S produced from the reaction between Rogor and NaOH solution. The experiment results showed that under the optimum coloring conditions 1×10 -6 Rogor pesticide could be detected at 390 nm absorption wavelength. The configuration method of the colorimetric system was analyzed and the optimum conditions were pH 5 7, suitable coloration temperature 24 ℃, and reaction time 20 s. The mathematical model was built by the least square method. The correlation coefficient was 0 941 5 and the sample recovery percent was 91 6%~108 9%, which provide the feasible basis for developing light source of portable pesticide residues detecting instrument.
Key words:  Rogor pesticide residues  colorimetry  spectrum analysis