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宫廷奶酪中优良乳酸菌菌株的分离鉴定
吴凤玉, 李 柳, 杨贞耐
0
(北京食品营养与人类健康高精尖创新中心)
摘要:
利用梯度稀释法和划线纯化法对4 种宫廷奶酪中的乳酸菌进行初筛,再通过产酸实验和过氧化氢酶实验进行复筛,得到对4 株乳酸菌。通过形态学特征观察和16S rDNA 序列分子鉴定,确定2 株为屎肠球菌(MRSA1、MRSD3),2 株为乳明串株菌(MRSA2、MRSB1)。抑菌实验表明乳明串珠菌MRSA2 对大肠杆菌、志贺氏菌、沙门氏菌具有良好的抑菌特;4 株菌均有抗氧化活性,菌悬液的DPPH 自由基清除能力和羟自由基清除能力均高于无细胞提取物,其中菌株MRSA2 菌悬液DPPH 自由基和羟自由基清除能力分别高达60.67%和31.86%。研究筛选出的具有良好抑菌特性和抗氧化活性的乳明串珠菌MRSA2 在功能性食品研发中具有潜在的应用价值。
关键词:  宫廷奶酪  分离  鉴定  乳明串珠菌
投稿时间:2018-11-14    修订日期:2019-01-24
基金项目:国家自然科学基金青年科学基金项目;江米酒凝乳酶分子特征及凝乳作用机制研究(31601488);2018年大学生科研与创业行动计划(201810011009)
Isolation and Identification of Lactic acid bacteria from traditional royal cheese
wufengyu, li liu, yangzhennai
(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,Beijing Technology & Business University)
Abstract:
In this study, four strains of lactic acid bacteria were obtained fromc four kinds of traditional royal cheese using gradient dilution method and scribing purification method, followed by re-screening using acid production experiment and catalase experiment. Through morphological observation and molecular identification of 16S rDNA identification sequence, two strains were identified as Enterococcus faecium (MRSA1, MRSD3), and the other two strains were identified as Leuconostoc lactis (MRSA2,MRSB1). The bacteriostatic test showed that Leuconostoc lactis(MRSA2)had good antibacterial properties against Escherichia coli, Shigella and Salmonella. All four strains had antioxidant activity, and the DPPH scavenging ability and hydroxyl radical scavenging ability of the bacterial suspension were higher than those of cell-free extracts. The DPPH and hydroxyl radical scavenging ability of MRSA2 suspension were as high as 60.67% and 31.86%, respectively. The strains of Lactic acid bacteria with antibacterial and antioxidative activities obtained in this study laid a foundation for their application in functional dairy foods.
Key words:  traditional royal cheese  isolation  identification  Leuconostoc lactis